BCF297 Laser Ukuskena iConfocal Microscopy
I-BCF297 yimakroskopu esanda kuqaliswa yokuskena i-laser, enokufikelela kuqwalaselo oluchanekileyo kunye nohlalutyo oluchanekileyo. Ingasetyenziswa ngokubanzi kwi-morphology, physiology, immunology, genetics kunye nezinye iinkalo. Liqabane elilungileyo lophando lwe-biomedical egqwesileyo.
Iimbonakalo
1. Umlinganiselo ophezulu wesignali ukuya kwingxolo.
Ubuchule obuphezulu bendlela yokubona i-confocal imaging inokubonelela ngemifanekiso ye-fluorescence enophawu oluphezulu kakhulu lwe-signal-to-noise ratio naphantsi kwe-fluorescence ebuthathaka.
2. Umfanekiso ogqwesileyo.
I-spectrum ebanzi, iilensi zokuvula amanani aphezulu, zilungele ukudubula iintlobo ezahlukeneyo zeesampulu ezidibeneyo.
3. Kulula ukuyisebenzisa.
Isakhelo sombane esigcweleyo, uyilo olulungisiweyo lojongano lokudityaniswa kwekhompyuter yomntu, ekuvumela ukuba wenze umsebenzi ngokulula ngexesha lokudubula isampulu.
4. Yonke iNkqubo yoLawulo lweemoto.
I-Z-axis ye-BCF297 ye-laser confocal microscope ithatha isixhobo esinemoto, esinokulungisa ngokukhawuleza ukuphakama kwe-Z-axis ngokomfanekiso wexesha langempela. I-AF One-iqhosha le-autofocus, ukuphelisa imfuno yamanyathelo okulungisa kakuhle kunye nokuphucula ukusebenza kakuhle.
Amaqhosha okulawula adibeneyo kumacala omabini omzimba anokuqonda ukutshintshwa ngokukhawuleza okanye ukujikeleza kwe-condenser, ukukhanya, i-lens yenjongo, i-turntable yefilimu yokunciphisa, kunye ne-fluorescence turntable, ephucula ukusebenziseka lula.
5. I-Superior Optics ye-Confocal Imaging.
(1) Isakhiwo se-pinhole ye-imaging ekhethekileyo: ukunciphisa ukuphazamiseka okubangelwa ukufuduka kwecandelo kunye nokuphucula umlinganiselo wesignali ukuya kwingxolo kunye nesisombululo se-axial somfanekiso.
(2) Iyunithi yokufumanisa umlawuli: iyunithi yokufumanisa uvakalelo oluphezulu (ubuninzi be-QE≥45%@500nm), enokugqiba ngokuzenzekelayo i-multi-color fluorescence confocal imaging ngokulula nangokukhawuleza.
6. Iiseti ezimbini zeelensi ezizikhethelayo.
I-BCF297 laser scanning confocal microscope ineeseti ezimbini zeelensi ezizikhethelayo, i-apochromatic (2X-100X) kunye ne-super-apochromatic (10X-100X), enoluhlu olubanzi lokugubungela, olulungele uphando oluphezulu lwemicroscope kunye nokudubula komfanekiso we-microscopic. Indawo enkulu yamanani iphucula ngakumbi isisombululo.
Isicwangciso esingapheliyo Injongo ye-APO
Isicwangciso esingapheliyo Injongo ye-APO
7. Isoftware ekhethekileyo ye-laser confocal.
Isoftware yale microscope edibeneyo igcwele iimpawu ezinamandla. I-BCF297 ixhasa i-channel eyodwa okanye i-multi-channel imaging ye-2D (XY), i-3D imaging (XYZ), i-4D imaging (XYZT) kunye ne-multi-site scanning. I-Image, i-photobleaching kunye ne-photostimulation inokwenziwa kwi-ROI echazwe ngumsebenzisi (iNgingqi yoMdla), i-Z-Stack imaging, ukuthungwa komfanekiso omkhulu, ukulungiswa kwesikali, ukulungiswa kwefayile, ukurekhoda idatha, njl.
Inkcazo
| Into | Inkcazo | BCF297 | |
| Umthombo wokukhanya weLaser | ILaser | 405nm/50mW, 488nm/50mW, 561nm/50mW, 640nm/40mW | ● |
| Ulawulo lweLaser | Ukumodareyitha ngokuthe ngqo kunye nokumodareyitha kwe-acousto-optic, zonke iiswitshi ze-laser kunye nobunzulu zinokuhlengahlengiswa ngokuthe ngqo, kwaye zinokungena ngokuzenzekelayo kwimeko yokucima ukuba ayisetyenziswanga ixesha elifutshane. | ● | |
| Skena imodyuli | Iyunithi yokuskena | Dual-axis XY high-speed optical scanning galvanometer | ● |
| Indawo yokujonga 14mm×14mm (≥ 19) | ● | ||
| Ukuskena ipixels 512×512～4096×4096 | ● | ||
| Ixesha lePixel 0.5μs ~ 8μs | ● | ||
| Isantya sokuskena esisemgangathweni: 1fps (512×512, 2μs) Isantya sokuskena ngokukhawuleza: 3fps (512×512, 0.5μs) | ● | ||
| Iskena sokwandisa: 1-32X | ● | ||
| Umngxuma wokuqhafaza | Ф30/40/50μm umbhobho ojikelezayo, ophakathi kwe-dichroic beam splitter kunye ne-galvanometer yokuskena, ukuqinisekisa ukuba ukuqokelela kwe-fluorescence ukusebenza kakuhle kwenkqubo kuhlala kugcinwa kwi-100% | ● | |
| Izibuko ze-Dichroic kunye nezihlungi | Isihlukanisi sentsimbi ye-dichroic ene-channel emine: 405/488/561/640nm | ● | |
| Ivili lokucoca elinemoto elinendawo emi-6 Liza kumgangatho ezine: 445nm/40, 530nm/43, 607nm/36 kunye ne685nm/40 | ● | ||
| Iyunithi yokufumanisa | MA PMT, QE ≥ 25%@500nm, 20%@600nm (PMT ubuninzi 1) | ● | |
| I-GaAsP PMT, QE ≥ 45%@500nm, 40%@600nm | ○ | ||
| Iyunithi yokufumanisa i-DIC | Ngomsebenzi wokucinga wophazamiseko olwahlukileyo, inokuqonda umfanekiso we- "DIC-fluorescence". | ● | |
| Imakroskopu yeBanga loPhando | Inkqubo yokubona | I-Infinity Chromatic Aberration eLungisiweyo ye-Optical System | ● |
| Intloko yoqwalaselo | I-20 ° -45 ° utyekelo olulungelelanisiweyo, umfanekiso oguqulweyo, infinity exhomekeke kwi-binocular observation ityhubhu, uluhlu lohlengahlengiso lwe-interpupillary: 50 ~ 76mm | ● | |
| Isixhobo samehlo | I-eyepiece ephezulu yeso ebanzi yeplani ye-eyepiece ye-PL10X / 22mm, i-diopter ehlengahlengiswayo, i-micrometer | ● | |
| Ilensi yenjongo | I-Infinity Plan Apochromat Iinjongo 4x, 10x, 20x, 40x, 60x, 100x | ● | |
| I-Infinity Plan Super Apochromat Objectives 10x, 20x, 40x, 60x, 100x | ○ | ||
| Isakhelo seMicroscope | Isikhundla esisezantsi se-coarse kunye ne-micro coaxial electric drive ekugxininiseni indlela, i-Z-axis stroke 10.5mm, echanekileyo 1um; phambi kobukhulu obukhulu bepaneli yokubonisa i-LCD, amaqhosha adibeneyo okulawula umbane emzimbeni, onokuqonda i-condenser, ukukhanya, i-lens yenjongo, i-optical port, njl. Ukutshintsha ngokukhawuleza phakathi kwemisebenzi. Izibuko elakhelwe ngaphakathi elinenjini, elikwicala lasekhohlo le-fuselage, umlinganiselo wokwahlula 100:0, 0:100; izibuko elakhelwe ngaphakathi elinemoto ekhohlo, umlinganiselo wokwahlula 0:100, 50:50, 100:0; I-single-layer / i-double-layer optical path optional , ukubonelela ngegumbi lokwandiswa kwenkqubo, kunye nokutshintshwa kweqhosha elilodwa kwiimodyuli zendlela ye-optical yoluhlu ngalunye ngokweemfuno. Kunye neVisor yeFluorescent, i-6-Position Motorized Converter (eneDIC Slot) kunye neKit yeAdapter yePort CTV | ● | |
| Iqonga | Iqonga lomatshini lokwenziwa ngesandla, ubungakanani betafile 300mm (X) × 240mm (Y), uluhlu oluhambayo 135mm (X) × 85mm (Y) Isiteji sombane, ubungakanani betafile obungekho ngaphantsi kwe-260mm (X) × 153mm (Y), uluhlu oluhambayo lwe-110mm (X) × 75mm (Y), kunye nesibambo sokusebenza esizimeleyo; isantya esiphezulu 3mm / S, phinda ukuchaneka kokumisa ± 1um; Ixhotyiswe nge-35mm petri dish, ukuqwalaselwa kwecandelo | ○ | |
| Icondenser | I-motorized 7-hole condenser, NA 0.55, WD 27mm; Imingxuma emi-3 ye-φ30mm (ukuchasana kwesigaba), imingxuma emi-4 ye-φ38mm (DIC); xhasa intsimi eqaqambileyo, umahluko wesigaba, ukujonga kwe-DIC (kubandakanywa neseti ye-polarizer) | ● | |
| Inkqubo yokukhanyisa ngeFluorescent | I-8-hole ye-fluorescent turntable system, inkqubo inokugqiba indawo ye-turntable ebekwe kwiindawo eziphezulu kunye nezantsi ze-rack; nayo inomsebenzi wokuvala umbane, onokuvala ngokuthe ngqo umthombo wokukhanyisa i-fluorescent, kunye neqela le-fluorescent filter block mirror group: B / G / UV, njl. | ● | |
| Isoftware esebenzayo
| Iskena umfanekiso | Ukufunyanwa komfanekiso kunye nomsebenzi wokulawula ngokuzenzekelayo, ukutshintshwa kombane ogcweleyo kwindlela yokubona; ukuziqhelanisa neeparamitha zekhamera kunye neeparamitha zokujonga kwangaphambili; intsimi epheleleyo yokujonga kunye nemifanekiso yokuskena ye-ROI; umfanekiso omnye we-2D wombala omnye okanye onemibala emininzi (XY), umfanekiso we-3D (XYZ), umfanekiso we-4D (XYZT) kunye nokuskena indawo ezininzi | ● |
| Uhlalutyo lokucubungula | Imibala emininzi ye-fluorescence colocalization processing, i-fluorescence kunye nokuphazamiseka kokuphazamiseka (DIC) umfanekiso ophezulu; ukulinganisa kunye nokongeza izikali; ukusetyenzwa kokucoca; Uhlalutyo lwe-Z-Stack processing kunye nokuthunga umfanekiso omkhulu | ● | |
| Ulawulo lwedatha | Iindidi ezahlukeneyo zoqhagamshelo lwezixhobo ziyakhethwa, iindlela zokugcina umfanekiso ngokuzenzekelayo zinokusetwa, iifomati ezininzi zemveliso yemifanekiso, kunye neeparamitha zokuskena zemifanekiso zinokugcinwa ngokuzenzekelayo. | ● | |
Qaphela: ●Isinxibo esiMiselweyo, ○Ngokuzikhethela
Imizekelo yeMifanekiso
Isatifikethi
Ulungiselelo
